Journal of bacteriology
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Sanada, Ichiro (Kanazawa University, Kanazawa, Japan), and Shoki Nishida. Isolation of Clostridium tetani from soil. J. ⋯ The glucose- and maltose-fermenting ability of these isolates was inversely proportional to their toxigenicity. The biological properties of atoxic strains were indistinguishable from those of C. tetanomorphum. Since a considerable number of toxic strains fermented glucose and maltose, these criteria are of doubtful value for differentiating C. tetani from C. tetanomorphum.
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Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. ⋯ A comparison of the value of this medium with other media in regard to toxin production by wild strains of C. novyi type A revealed that in this medium the organism could produce more consistent yields of potent toxin than in the other media. Isolation of C. novyi was attempted from 62 soil samples, and all were found to contain this organism. Toxigenicities of strains isolated under various conditions were examined in the above-mentioned medium, with the following results. (i) The longer the duration of the sample incubation, the less toxigenic were the strains isolated. (ii) When higher temperatures were employed to preheat the sample, fewer toxigenic strains were obtained.
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Journal of bacteriology · Dec 1964
TAXONOMY OF CLOSTRIDIUM BIFERMENTANS AND CLOSTRIDIUM SORDELLII. I. THEIR TOXIGENICITY, UREASE ACTIVITY, AND SPORULATING POTENCY.
Nishida, S. (Kanazawa University, Kanazawa, Japan), K. Tamai, and T. Yamagishi. ⋯ Substrains of typical cultures of C. sordellii thus can yield either substrains resembling nonpathogenic strains of C. sordellii culturally, or substrains resembling C. bifermentans. The sporulating potency of pathogenic and nonpathogenic strains of C. sordellii and strains of C. bifermentans proved to be significantly distinct. The sporulating potency of C. bifermentans was high, that of pathogenic strains of C. sordellii was low, and that of nonpathogenic strains of C. sordellii was intermediate between the other two.
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Journal of bacteriology · Dec 1964
TAXONOMY OF CLOSTRIDIUM BIFERMENTANS AND CLOSTRIDIUM SORDELLII. II. TOXIGENIC AND SPORULATING POTENCIES IN SUBSTRAINS OF A CLOSTRIDIUM SORDELLII STRAIN.
Tamai, Kenzo (Kanazawa University, Kanazawa, Japan), and Shoki Nishida. Taxonomy of Clostridium bifermentans and Clostridium sordellii. II. ⋯ The difference in the six criteria gradually disappeared as the sporulating potency of the substrains of C. sordellii 4708 was strengthened. The substrains which could resist heating at 90 C for 10, 20, or 30 min were found to have lost all six criteria for distinction and were biologically in agreement with C. bifermentans. We further demonstrated that all newly isolated strains of C. bifermentans examined possessed extremely strong sporulating potency.
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Journal of bacteriology · Sep 1964
ISOLATION OF TOXIGENIC STRAINS OF CLOSTRIDIUM PERFRINGENS FROM SOIL.
Yamagishi, Takayoshi (Kanazawa University, Kanazawa, Japan), Shoichi Ishida, and Shoki Nishida. Isolation of toxigenic strains of Clostridium perfringens from soil. J. ⋯ Toxigenic strains could not be obtained from soil specimens heated at 100 C for 60 min. All toxigenic strains were killed by heating at 100 C for 10 min; strains that were resistant to these conditions were found to be nontoxigenic. These findings suggest that sporulating ability is closely linked to toxigenicity.