Letters in applied microbiology
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During aseptic manufacturing and specifically during the transfer of items into an isolator, disinfection of surfaces is essential for reducing the risk of final product contamination. Surface disinfection can be carried out by a variety of methods, however the most accepted current practice is a combination of spraying with 70% alcohol and wiping. The aim of this study was to evaluate the effectiveness of two wipe systems by determining their ability to remove, kill and transfer bacterial contaminants from standardized surfaces. ⋯ The impregnated wipes performed better than the dry wipes sprayed with alcohol and should be used for surface disinfection in aseptic units.
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Lett. Appl. Microbiol. · Jan 2003
Quantification of Listeria spp. contamination on shell and flesh of cooked black tiger prawns (Penaeus monodon).
To quantify Listeria levels on the shell and flesh of artificially contaminated cooked prawns after peeling, and determine the efficacy of Listeria innocua as a model for L. monocytogenes in this system. ⋯ Reduced risk from L. monocytogenes on prawns can only be assured by adequate hygiene or heating.
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Lett. Appl. Microbiol. · Nov 1998
Mycobacterium tuberculosis complex DNA in calcified pleura from remains 1400 years old.
Mycobacterium tuberculosis complex DNA was isolated and identified in calcified pleura from remains 1400 years old, with the polymerase chain reaction. This is the first demonstration of tuberculosis in non-mummified archaeological tissue other than bone; the presence of mycobacterial mycolic acids in the sample supports this conclusion. The study of ancient DNA from microbial pathogens is of interest as it enables verification of traditional diagnoses, may answer long-standing questions in the history of disease, and provides ancient DNA sequences that can be compared with those of modern isolates.
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Lett. Appl. Microbiol. · Sep 1996
Effect of fungistatic agent 2-deoxy-D-glucose on mycotoxins from Penicillium expansum.
Mycotoxin production from Penicillium expansum was effected by the 'biocontrol enhancer' 2-deoxy-D-glucose (DOG). Citrinin and gentisyl alcohol were produced in higher amounts, whereas patulin was inhibited, which represents a differential effect on the polyketide metabolic pathway. These effects have implications for the use of DOG in biocontrol.