American journal of respiratory and critical care medicine
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Am. J. Respir. Crit. Care Med. · Feb 2021
In Vivo Generation of Lung and Thyroid Tissues from Embryonic Stem Cells using Blastocyst Complementation.
Rationale: The regeneration and replacement of lung cells or tissues from induced pluripotent stem cell- or embryonic stem cell-derived cells represent future therapies for life-threatening pulmonary disorders but are limited by technical challenges to produce highly differentiated cells able to maintain lung function. Functional lung tissue-containing airways, alveoli, vasculature, and stroma have never been produced via directed differentiation of embryonic stem cells (ESCs) or induced pluripotent stem cells. We sought to produce all tissue components of the lung from bronchi to alveoli by embryo complementation. ⋯ ESC-derived cells from multiple respiratory cell lineages were highly differentiated and indistinguishable from endogenous cells based on morphology, ultrastructure, gene expression signatures, and cell surface proteins used to identify cell types by fluorescence-activated cell sorter. Conclusions: Lung and thyroid tissues were generated in vivo from ESCs by blastocyst complementation. Nkx2-1-/- chimeras can be used as "bioreactors" for in vivo differentiation and functional studies of ESC-derived progenitor cells.
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Am. J. Respir. Crit. Care Med. · Feb 2021
Letter Observational StudyUrine Titin N-fragment as a Biomarker of Muscle Injury for Critical Illness Myopathy.