Journal of investigative medicine : the official publication of the American Federation for Clinical Research
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To investigate the effort of mitochondrial calcium transport and calcium-induced membrane permeability transition in alleviating atherosclerosis. The experimental mice were divided into three groups: the control group (C57BL/6 mice with normal diet), the atherosclerosis group (apolipoprotein E-deficient (ApoE-/-) mice with high-fat diet) and the mitochondrial targeting agent group (ApoE-/- mouse with high-fat diet). The mean fluorescence intensity of Ca2+ in the atherosclerosis group is significantly higher than control group and mitochondrial targeting agent group. ⋯ The macrophage recruitment (F4/80 positive area) and the expression of tumor necrosis factor alpha, interleukin-6, pyrin domain containing protein 3, intercellular cell adhesion molecule-1, p38 mitogen-activated protein kinase and Jun kinase 1/2 phosphorylation in the atherosclerosis group are higher that other groups. Treatment with mitochondrial targeting agents reduced the levels of elevated cyt C and cleaved caspase-3 in atherosclerotic mice (p<0.05). Mitochondrial targeting agents interfere with mitochondrial calcium transport and calcium-induced membrane permeability transition, inhibit MAPK/JNK pathway activation, inhibit foam cell formation and alleviate the process of atherosclerosis.
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How to give full play to the positive function of constructive conflicts (task conflicts and process conflicts) in the science and technology innovation team, give appropriate stimulation, mobilize the enthusiasm of employees and improve the team's innovative ability to improve team performance and organizational effectiveness are issues that deserve both team leaders' and scholars' attention. Through selecting multiple medical technology R&D personnel from a specific science and technology innovation team of health organization, the research studies the constructive conflicts among the members of the science and technology innovation team, constructs and analyzes the conflict evolution game model, proposes countermeasures and suggestions for improving the innovation ability of the science and technology innovation team and discusses the innovation management mechanism of the science and technology innovation team. ⋯ However, on the one hand, constructive conflicts can be controlled to maintain a moderate state of control. On the other hand, it is also necessary to establish a mutually trusting communication environment and convenient communication channels in the science and technology innovation team, combined with modern information management technology, to handle the problems that were difficult to be found or accumulated for a long time under the previous management mechanism and cooperate with the science and technology innovation team to improve the technology innovation team's innovation capability.
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Previous animal models of gastroesophageal reflux disease (GERD) were not physiological and required a variety of surgical procedures. Therefore, the animal model developed by conditions that are similar to the pathogenesis of GERD is necessary. The aim is to establish a non-surgical animal model with GERD caused by overeating induced in mice. ⋯ The higher frequency of fasting and overeating could cause GERD effectively. The dietary control can make mice overeat, which elicits the change of lower esophageal mucosa similar to GERD. Thus, the overeating-induced mouse may be used as a GERD mouse model.