Pak J Med Sci
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To analyze the clinicopathological features and risk factors of Type-2 diabetes mellitus (T2DM) patients with non-alcoholic fatty liver disease (NAFLD). ⋯ High BMI, BPG, insulin resistance index and TG are independent risk factors for nonalcoholic fatty liver in T2DM patients. During intervention, attention should be paid to the monitoring of these indicators to effectively prevent the aggravation of the disease.
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Extraction of DNA and RNA is the first step in genomics and transcriptomics studies. Phenol-chloroform method for DNA extraction has been the widely used method. However, this method is relatively expensive and time-consuming. The objective of the present study was to validate a cost and time-effective protocol that will reduce the burden of molecular biology-based research and make a difference in laboratories with limited resources. ⋯ The TRIzol™ method for RNA and DNA co-extraction is fast, simple and economical technique. So, it can be adopted for routine molecular biology analyses in limited resources setup.
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Obesity is a global health problem, and obese patients are subject to developing abdominal wall hernias. There are few prospective studies comparing the laparoscopic method of umbilical hernia mesh repair between abdominal obesity patients and normal abdominal waist patients. The aim of this study was to evaluate the short-term outcomes (operative time, early complications and hospital stay) in the patients having laparoscopic hernia repair with abdominal obesity. ⋯ Laparoscopic umbilical hernia repair can be safely performed in abdominal obesity in male patients without an additional risk of complications.
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To analyze the distribution of common pathogenic bacteria and pattern of drug resistance in the blood culture of inpatients. ⋯ G-positive bacteria were mainly detected in the positive blood culture samples of inpatients in this hospital. Daptomycin, linezolid and vancomycin were preferred for G-positive cocci, while amikacin was highly sensitive to G-negative bacilli.
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To determine the carboxyhemoglobin (COHb) levels of the patients admitted to the pediatric intensive care unit and investigate its relationship with prognosis. ⋯ Since SpCO levels are increased in critically ill children and correlate with increased PICU mortality, SpCO may be a predictive marker for prognosis in PICU.