Articles: cations.
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DNA origamis are fully tailored, programmable, biocompatible and readily functionalizable nanostructures that provide an excellent foundation for the development of sophisticated drug-delivery systems. However, the DNA origami objects suffer from certain drawbacks such as low cell-transfection rates and low stability. A great deal of studies on polymer-based transfection agents, mainly focusing on polyplex formation and toxicity, exists. ⋯ Besides brick-like DNA origamis, tubular origami carriers equipped with enzymes were also coated with the polymers. By adjusting the amount of cationic polymers that cover the DNA structures, we showed that it is possible to control the enzyme kinetics of the complexes. This work gives a starting point for further development of biocompatible and effective polycation-based block copolymers that can be used in coating different DNA origami nanostructures for various bioapplications.
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The development of colorectal cancer is influenced by hormonal factors. Oophorectomy alters endogenous levels of sex hormones, but the effect on colorectal cancer risk is unclear. The aim of this cohort study was to examine colorectal cancer risk after oophorectomy for benign indications. ⋯ Colorectal cancer risk is increased after oophorectomy for benign indications.
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Normal pressure hydrocephalus frequently develops after subarachnoid hemorrhage. It can often be difficult, however, to predict shunt dependency. The purpose of this study was to identify predictors of shunt-dependent normal pressure hydrocephalus (SDNPH) after aneurysmal subarachnoid hemorrhage (aSAH). ⋯ SDNPH after aSAH in our study showed no correlations with three of the parameters previously identified as risk factors for shunt-dependent hydrocephalus, namely, the amount of SAH, the presence of IVH, or acute hydrocephalus. Instead, a longer duration of CSF drainage correlated with SDNPH as an independent factor. These data suggest that a longer duration of CSF drainage may be one of the risk factors for SDNPH after aSAH.
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Damage to the motor division of the lower cranial nerves that run into the jugular foramen leads to hoarseness, dysphagia, and the risk of aspiration pneumonia; therefore, its functional preservation during surgical procedures is important. Intraoperative mapping and monitoring of the motor rootlets at the cerebellomedullary cistern using endotracheal tube electrodes is a safe and effective procedure to prevent its injury. ⋯ EMG, electromyographic.