• Gac Med Mex · Jan 2019

    Identificación de Mycobacterium bovis en pacientes con diagnóstico de tuberculosis pulmonar y extrapulmonar.

    • Alejandro Hernández-Solís, Maribel González-Villa, Raúl Cícero-Sabido, Heleodora González-González, Yesenia Colin-Muñoz, Alejandra Camerino-Guerrero, and Ernesto Ramírez-González.
    • Secretaría de Salud, Hospital General de México "Dr. Eduardo Liceaga", Unidad de Neumología. Ciudad de México, México.
    • Gac Med Mex. 2019 Jan 1; 155 (6): 608-612.

    IntroductionIn Mexico, there is an alarming increase in the number of cases of Mycobacterium bovis infection on pulmonary and extrapulmonary presentations. The lack of timely identification triggers complications and increases mortality.ObjectiveTo know the frequency of M. bovis infections in clinical samples of patients with tuberculosis in the mycobacteria laboratory of a reference hospital in Mexico City.MethodProspective, descriptive study. Strains isolated from biological material were studied in Löwestein-Jensen and MGITI960 cultures. M. bovis was identified by amplifying the RD9 fragment with end-point polymerase chain reaction (PCR).ResultsEight-hundred and fifty tuberculosis-diagnosed patients were included; in 441 cases, Mycobacterium tuberculosis was confirmed by positive culture (250 pulmonary, 65 ganglionic, 39 renal, 34 meningeal, 25 miliary, 14 pleural, 8 peritoneal, 4 bone and 2 pericardial cases). Forty-eight strains (10.8%) were typified as M. bovis by amplification of the RD9 fragment with end-point PCR.ConclusionsM. bovis is not currently thought of a causative agent of tuberculosis, which could be the cause of pharmacological treatment failure. In this study, the main extrapulmonary form was observed to be cervical lymphadenopathy.Copyright: © 2019 Permanyer.

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