• J. Clin. Virol. · Aug 2020

    Comparative Study

    Comparison of commercial lateral flow immunoassays and ELISA for SARS-CoV-2 antibody detection.

    • Maria Martínez Serrano, David Navalpotro Rodríguez, Nuria Tormo Palop, Roberto Olmos Arenas, Marta Moreno Córdoba, Mª Dolores Ocete Mochón, and Concepción Gimeno Cardona.
    • Microbiology Department, Consorcio Hospital General Universitario. Address: Av. Tres Cruces s/n, 46014, Valencia, Spain. Electronic address: martinez_mariaser@gva.es.
    • J. Clin. Virol. 2020 Aug 1; 129: 104529.

    BackgroundCOVID-19 pandemic has spread worldwide since December 2019. Serological tests for SARS-CoV-2 antibody testing are needed for detection of current or past infections. A wide range of commercial tests is available. However, most of them need to be validated.Study DesignThe aim was to compare a commercial IgG and IgA ELISA (Euroimmun) with three lateral flow immunoassays (LFI): Hangzhou Alltest Biotech, Wuhan UNscience Biotechnology and Guangzhou Wondfo Biotech. Specificity was calculated with 62 available serum samples from 2018/19. The study included 152 sera from patients of which 109 were RT-PCR positive. Sensitivities for ELISA anti SARS-CoV-2 IgG and IgA were 81.5 % and 93.1 % and specificities 100 % and 80.6 %, respectively. LFI showed variable performances, overall results being better for Guangzhou Wondfo Biotech.ConclusionsCommercial serological tests are useful for detection of antibodies in patients with COVID-19. ELISA presented better results than LFI. The results allowed to incorporate the most sensitive LFI to the daily workflow, combining with ELISA. Careful validation is encouraged before clinical laboratories start using these tests.Copyright © 2020 Elsevier B.V. All rights reserved.

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