• Eur J Nutr · Feb 2016

    Effect of neonatal malnutrition on expression of nitric oxide synthase enzyme, production of free radicals and in vitro viability of alveolar macrophages infected with methicillin-sensitive and methicillin-resistant Staphylococcus aureus.

    • de Morais Natália Gomes NG Health Sciences Center, Federal University of São Francisco Valley (UNIVASF), University Campus, Paulo Afonso, BA, 48608-170, Brazil. morais.ng, da Costa Thacianna Barreto TB Department of Tropical Medicine, Federal University of Pernambuco (UFPE), University Campus, University Town, Recife, PE, 50670-420, Brazil., Amanda Lúcia Farias Pedrosa, Maria Carolina Accioly Brelaz de Castro, Suênia Cunha da Gonçalves de Albuquerque, Pereira Valéria Rêgo Alves VR The Laboratory of Immunogenetics, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation - CPqAM/FIOCRUZ, Campus UFPE, Recife, Brazil., Milena de Paiva Cavalcanti, and Célia Maria Machado Barbosa de Castro.
    • Health Sciences Center, Federal University of São Francisco Valley (UNIVASF), University Campus, Paulo Afonso, BA, 48608-170, Brazil. morais.ngm@gmail.com.
    • Eur J Nutr. 2016 Feb 1; 55 (1): 403-11.

    ObjectiveEvaluate the effects of neonatal malnutrition on the microbicidal response and viability of in vitro macrophages infected with Staphylococcus aureus sensitive/resistant to methicillin.MethodsMale Wistar rats (n = 24) were divided into two distinct groups: nourished (rats breast-fed by mothers undergoing diet with 17% casein) and malnourished (rats breast-fed by mothers undergoing diet with 8% casein). Macrophages were recovered after surgical tracheostomy procedure by collecting bronchoalveolar lavage. Four systems were established: negative control, composed only by phagocytes; positive control, macrophages plus lipopolysaccharide; and two test systems, macrophages plus Staphylococcus aureus sensitive and resistant to methicillin. Plates were incubated at 37 °C for 24 h. After this period, tests for the analysis of cell viability and microbicidal response were performed. In the statistical analysis, the Student's t and ANOVA tests were used, accepting p < 0.05.ResultsThe neonatal malnutrition impaired the animals' body weight. There was a lower expression of the inducible nitric oxide enzyme (iNOS), nitric oxide production, and viability of macrophages infected with methicillin-resistant Staphylococcus aureus. However, increased production of superoxide anion in the malnourished group was detected.ConclusionNeonatal malnutrition focusing on critical periods of development promoted lower expression of iNOS, nitric oxide production, cell viability, and exacerbated reactive oxygen species production. The high levels of reactive oxygen species may favor the onset of serious and systemic infections with fatal outcome if associated with methicillin-resistant Staphylococcus aureus.

      Pubmed     Full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        
    You can also include formatting, links, images and footnotes in your notes
    • Simple formatting can be added to notes, such as *italics*, _underline_ or **bold**.
    • Superscript can be denoted by <sup>text</sup> and subscript <sub>text</sub>.
    • Numbered or bulleted lists can be created using either numbered lines 1. 2. 3., hyphens - or asterisks *.
    • Links can be included with: [my link to pubmed](http://pubmed.com)
    • Images can be included with: ![alt text](https://bestmedicaljournal.com/study_graph.jpg "Image Title Text")
    • For footnotes use [^1](This is a footnote.) inline.
    • Or use an inline reference [^1] to refer to a longer footnote elseweher in the document [^1]: This is a long footnote..

    hide…