• Sine Wichmann, Gitte Færk, Jens R Bundgaard, and Mona R Gätke.
• aDanish Cholinesterase Research Unit, Department of Anaesthesiology, Herlev and Gentofte Hospital, University of Copenhagen bDepartment of Clinical Biochemistry, Herlev and Gentofte Hospital, Herlev cDepartment of Clinical Biochemistry, University of Copenhagen, Copenhagen, Denmark.
• Pharmacogenet. Genomics. 2016 Jul 1; 26 (7): 351-6.

IntroductionMutations in the butyrylcholinesterase enzyme (BChE) can result in prolonged duration of action of the neuromuscular blocking agents, succinylcholine and mivacurium, as BChE hydrolyses these drugs. Hereditary low BChE activity can cause extensively prolonged apnoea during general anaesthesia when these drugs are used. The aim of this study was to describe novel mutations in the butyrylcholinesterase gene (BCHE) in patients who have experienced prolonged duration of action of mivacurium or succinylcholine.MethodsThe Danish Cholinesterase Research Unit registers patients with prolonged duration of action to succinylcholine and mivacurium. Patients were studied if they had equivocal phenotypes on the basis of BChE activity, biochemical inhibitor reactions and with pedigree if possible. Complete nucleotide sequencing was performed to describe the genotype and pedigree was used to separate the alleles. Multiple sequence alignment of BChE was performed for comparison with other species.ResultsGenotyping indicated seven novel mutations in the BCHE (I373T, G467S, W518R, L184S, V421A, M462I and R577H).ConclusionWe have found seven new variants of the BCHE, which seem to reduce the activity of BChE in patients undergoing anaesthesia involving succinylcholine or mivacurium.

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