• Zhonghua Yan Ke Za Zhi · Nov 1999

    [Effect of bFGF, IGF-I and their synergy on the mitogenesis of bovine lens epithelial cells].

    • Z Sun, K Yao, R Wu, X Shentu, and W Xu.
    • Department of Ophthalmology, The Second Affiliated Hospital of Zhejiang Medical University, Hangzhou 310009.
    • Zhonghua Yan Ke Za Zhi. 1999 Nov 1; 35 (6): 462-4.

    ObjectiveTo assess the effect of basic fibroblast growth factor (bFGF), insulin like growth factor-I (IGF-I) and their synergy on the mitosis of bovine lens epithelial cells (BLECs).MethodsBLECs were cultured in vitro. The fourth passage cells were inoculated into 24 well tissue culture plates, treated with different concentrations of bFGF, IGF-I, and bFGF + 20 microg/L IGF-I for 20 hours, (3)H-thymidine was added for 16 hours, then the radioactivity was determined by liquid scintillation counting.ResultsEither bFGF (1 - 100 microg/L) or IGF-I (20 - 100 microg/L) increases mitosis of BLEC significantly, and 20 microg/L IGF-I may increase bFGF induced mitogenesis.ConclusionGrowth factor and their synergic effect may play an important role in lens epithelial cell proliferation.

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