• Zhengtu Li, Yinhu Li, Lingdan Chen, Shaoqiang Li, Le Yu, Airu Zhu, Feng Yang, Qian Jiang, Liyan Chen, Jincun Zhao, Wenju Lu, Nanshan Zhong, and Feng Ye.
• State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
• Clin. Infect. Dis. 2021 Jul 15; 73 (2): e426-e433.

BackgroundSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2.MethodsHowever, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR.ResultsThis case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found.ConclusionsThis case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis.© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

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