• T N Pirvu, J E Schroeder, M Peroglio, S Verrier, L Kaplan, R G Richards, M Alini, and S Grad.
• AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos, Switzerland.
• Eur Spine J. 2014 Apr 1; 23 (4): 745-53.

PurposePlatelet-rich plasma (PRP) contains growth factors and creates a 3D structure upon clotting; PRP or platelet lysate (PL) might be considered for annulus fibrosus (AF) repair.MethodsBovine AF cells were cultured with 25% PRP, 50% PRP, 25% PL, 50% PL, or 10% FBS. After 2 and 4 days, DNA, glycosaminoglycan (GAG), and mRNA levels were analyzed. Histology was performed after injection of PRP into an AF defect in a whole disc ex vivo.ResultsBy day 4, significant increases in DNA content were observed in all treatment groups. All groups also showed elevated GAG synthesis, with highest amounts at 50% PL. Collagen I and II expression was similar between groups; aggrecan, decorin, and versican expression was highest at 25% PL. Injection of PRP into the AF defect resulted in an increased matrix synthesis.ConclusionsPlatelet-rich preparations increased the matrix production and cell number and may therefore be considered to promote AF repair.

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