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Archives of virology · Oct 2020
Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.
- Mariano Carossino, Hon S Ip, Juergen A Richt, Kendra Shultz, Kimberly Harper, Alan T Loynachan, Fabio Del Piero, and Udeni B R Balasuriya.
- Louisiana Animal Disease Diagnostic Laboratory, Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, River Rd, Room 1043, Baton Rouge, LA, 70803, USA.
- Arch. Virol. 2020 Oct 1; 165 (10): 2373-2377.
AbstractIn situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.
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