• J. Clin. Virol. · Feb 2008

    Rapid detection of important human pathogenic Phleboviruses.

    • Manfred Weidmann, M Paz Sanchez-Seco, Amadou A Sall, Peinda Ogo Ly, Yaya Thiongane, Modou M Lô, Heike Schley, and Frank T Hufert.
    • Institute of Virology, University of Göttingen, Kreuzbergring 57, 37075 Göttingen, Germany. mweidma@gwdg.de
    • J. Clin. Virol. 2008 Feb 1; 41 (2): 138-42.

    BackgroundRapid diagnostics are not available for several human pathogens in the genus Phlebovirus of the Bunyaviridae.ObjectivesTo develop RT-PCR assays for Sandfly Fever Sicilian virus (SFSV), Sandfly Fever Naples virus (SFNV), Toscana virus (TOSV) and Rift Valley Fever virus (RVFV).Study DesignRNA standards were generated and used to test the performance of the assays.ResultsA detection limit of 10-100 RNA molecules was determined for the SFSV, TOSV and RVFV assays. The sensitivity of the SFNV assay was not determined. The TOSV and the RVFV assays detected recent isolates from Spain and Africa, respectively.ConclusionThe assays should help to improve surveillance of pathogenic Phleboviruses.

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