• J. Am. Coll. Surg. · Jul 2013

    A true orthotopic gastric cancer murine model using electrocoagulation.

    • Jasneet Singh Bhullar, Tafadzwa Makarawo, Gokulakkrishna Subhas, Ahmed Alomari, Boris Silberberg, Jacqueline Tilak, Milessa Decker, and Vijay K Mittal.
    • Department of Surgery, Providence Hospital and Medical Centers, Southfield, MI 48075, USA. drjsbhullar@gmail.com
    • J. Am. Coll. Surg.. 2013 Jul 1;217(1):64-70; discussion 70-1.

    BackgroundOrthotopic mouse models of human gastric cancer represent an important in vivo tool for testing chemotherapeutic agents and for studying intraluminal factors. Currently, orthotopic mouse models of gastric cancer require an operative procedure involving either injection or implantation of tumor cells in stomach layers. The resultant tumor does not grow from the stomach's mucosal surface, so it does not mimic the human disease process.Study DesignA low-dose gastric mucosal coagulation was done transorally in the body of stomach using a specially designed polyethylene catheter in 16 female severe combined immunodeficient mice. This was followed by the instillation of SNU-16 human gastric cancer tumor cells (1 × 10(6) cells). Five mice each were euthanized at 1 and 2 months, and 6 mice were euthanized at 3 months. Three control mice underwent electrocoagulation alone and 3 mice underwent cell line instillation alone.ResultsTumors were detected in 11 of 16 experimental mice, but not in the control mice. Tumors were noted in mice at 1 month. Over time, there was an increase in tumor growth and metastasis to lymph nodes and surrounding organs. Histopathologic evaluation showed that the tumors grew from the gastric mucosa.ConclusionsOur model is easy to create and overcomes the limitations of the existing models, as the tumor arises from the stomach's mucosal layer and mimics the human disease in terms of morphology and biologic behavior. This is the first report of a true orthotopic gastric cancer murine model. This model opens new doors for additional studies that were not possible earlier.Copyright © 2013 American College of Surgeons. Published by Elsevier Inc. All rights reserved.

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