• Ming Zhang, Hongxia Zheng, Xiaolu Zhang, Xiaoli Tian, Shengdi Xu, You Liu, Shuyuan Jiang, Xiaolei Liu, Rui Shi, Kerui Gong, Shaochun Yan, He Wang, Guo Shao, and Zhanjun Yang.
• a Biomedicine Research Center and Basic Medical College , Baotou Medical College , Inner Mongolia , PRC.
• J Toxicol Env Heal A. 2018 Jan 1; 81 (21): 1116-1122.

AbstractDNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of neural stem cells into neurons. The aim of this study was to determine whether nerve growth factor (NGF) was involved in differentiation of mouse hippocampal neuronal cell line (HT22) as assessed by IncuCyte. Quantitative PCR and western blot were used to measure gene and protein expression of DNMT as well as the activity of DNMTs. Treatment with NGF was found to upregulate both gene and protein expressions as well as total activity of DNMTs in differentiating HT22 cells. Compared to undifferentiating cells, the percentage of differentiating cells at S phase increased significantly when incubated with NGF. In undifferentiated cells, NGF failed to induce gene and protein expressions and activity of DNMTs. Data demonstrate that differentiation of HT22 cells by exposure to NGF involve the activation of DNMTs pathway.

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