• Adv Exp Med Biol · Jan 2016

    Review

    Mass Spectrometry-Based Protein Quantification.

    • Yun Chen, Fuqiang Wang, Feifei Xu, and Ting Yang.
    • School of Pharmacy, Nanjing Medical University, 818 Tian Yuan East Road, Nanjing, 211166, China. ychen@njmu.edu.
    • Adv Exp Med Biol. 2016 Jan 1; 919: 255-279.

    AbstractQuantification of individual proteins and even entire proteomes is an important theme in proteomics research. Quantitative proteomics is an approach to obtain quantitative information about proteins in a sample. Compared to qualitative or semi-quantitative proteomics, this approach can provide more insight into the effects of a specific stimulus, such as a change in the expression level of a protein and its posttranslational modifications, or to a panel of proposed biomarkers in a given disease state. Proteomics methodologies, along with a variety of bioinformatics approaches, are a major tool in quantitative proteomics. As the theory and technological aspects underlying the proteomics methodologies will be extensively described in Chap. 20 , and protein identification as a prerequisite of quantification has been discussed in Chap. 17 , we will focus on the quantitative proteomics bioinformatics algorithms and software tools in this chapter. Our goal is to provide researchers and newcomers a rational framework to select suitable bioinformatics tools for data analysis, interpretation, and integration in protein quantification. Before doing so, a brief overview of quantitative proteomics is provided.

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