• Methods Mol. Biol. · Jan 2009

    High accuracy mass spectrometry in large-scale analysis of protein phosphorylation.

    • Jesper V Olsen and Boris Macek.
    • Department of Proteomics and Signal Transduction, Max Planck Institute for Biochemistry, Martinsried, Germany.
    • Methods Mol. Biol. 2009 Jan 1; 492: 131-42.

    AbstractWith the appearance of a new generation of high-performance hybrid mass spectrometers, high accuracy (sub-parts-per-million) mass spectrometry is becoming increasingly available to a wider scientific community. Here we discuss the advantages of such mass spectrometric instrumentation in the global analysis of protein phosphorylation. We describe a detailed workflow for fractionation and enrichment of phosphopeptides from digests of whole cell/tissue lysates by strong cation exchange and TiO(2) chromatography and their subsequent measurement on an LTQ-Orbitrap mass spectrometer under several acquisition regimes.

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