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Zhong Nan Da Xue Xue Bao Yi Xue Ban · Aug 2011
Oridonin induced the apoptosis of PC-3 cells and its mechanism.
- Jin Li, Luoyan Yang, and Hongtao Wu.
- Department of Urology, Central Hospital of Xiangtan, Xiangtan Hunan 411100,China.
- Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Aug 1; 36 (8): 754-9.
ObjectiveTo observe the proliferation inhibition and apoptosis promotion effect of oridonin on PC-3 cells.MethodsPC-3 cells were treated with different concentrations of oridonin. MTT assay and drug concentration-time survival curve were used to test the effect of oridonin on the PC-3 cells. The percentage of earlier apoptosis cells was analyzed by flow cytometry. The protein expression of caspase-3, Bcl-2, and Bax in the PC-3 cells was detected by Western blot.ResultsOridonin effectively inhibited the proliferation of PC-3 cells in both concentration- and time-dependent manner, and the IC50 of PC-3 cells was 10.29 μmol/L. HochesT(3)3258 staining and flow cytometry deteced that oridonin induced the apoptosis of PC-3 cells in a concentration-dependent manner (P<0.05). Oridonin down-regulated Bcl-2, up-regulated Bax protein, and activated caspase-3 in a concentration-dependent manner in the PC-3 cells.ConclusionThe apoptosis of PC-3 cells induced by oridonin might be associated with the mitochondrial pathway.
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