• Abbas Raisi, Saeed Azizi, Nowruz Delirezh, Behnam Heshmatian, Amir Abbas Farshid, and Keyvan Amini.
• From the Departments of Clinical Sciences (A.R., S.A.), and Pathobiology (A.A.F.), Faculty of Veterinary Medicine, and Department of Cellular and Molecular Biotechnology (N.D.), Institute of Biotechnology, Urmia University; and Neurophysiology Research Center (B.H.), Urmia University of Medical Sciences, Urmia, Iran; and Department of Veterinary Pathology (K.A.), Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
• J Trauma Acute Care Surg. 2014 Apr 1; 76 (4): 991-7.

BackgroundThe accomplishment for desired functional peripheral nerve regeneration is still challenging despite various materials and methods. The effects of local application of omental adipose mesenchymal stromal cell-derived microvesicles (MVs) on peripheral nerve regeneration were studied using a rat sciatic nerve transection model.MethodsA 10-mm gap of sciatic nerve was bridged with a chitosan conduit. The rats were divided into five experimental groups randomly as follows: cultured undifferentiated omental adipose-derived stromal cells, rest mesenchymal stem cell-derived MVs (c-MVs), anti-inflammatory mesenchymal stem cell-derived MVs (anti-MVs), proinflammatory mesenchymal stem cell-derived MVs (pro-MVs), and negative control (Chit).ResultsThe functional assessment of nerve regeneration (walking track analyses), electrophysiologic measurements, muscle mass measurements, as well as histomorphometrical and immunohistochemical indices showed drastic improvement in nerve regeneration in c-MVs and anti-MVs animals compared with pro-MVs animals (p < 0.05).ConclusionThe anti-inflammatory stem cell-derived MVs can be used as an alternative for the improvement of rat sciatic nerve regeneration.

23 keywords...

hide…