• Mehmet Aziret, Gamze Güney Eskiler, Gözde Çakırsoy Çakar, Asuman Deveci Özkan, Metin Ercan, Cemil Bilir, Erdal Polat, Havva Belma Koçer, Ebru Kayra Yıldırım, and Mustafa Duman.
• University of Health Science, Balıkesir City Education and Research Hospital, Balıkesir, Turkey; Sakarya University Faculty of Medicine, Department of General Surgery, Sakarya, Turkey. Electronic address: mhmtaziret@gmail.com.
• Clinics (Sao Paulo). 2023 Jan 1; 78: 100271100271.

AimThis study aimed to evaluate the expression levels of miR-99b and miR-135b in peritoneal carcinoma and liver metastases associated with Colorectal Cancer (CRC), assess their association with the intracellular signaling pathway proteins Kirsten Rat Sarcoma Virus (KRAS) and Akt, and investigate their effects on survival.Materials And MethodsChanges in the KRAS gene and Akt proteins, expression levels of miR-99b and miR-135b, and factors affecting survival were compared between colorectal cancer-associated peritoneal carcinomatosis and liver metastasis.ResultsThe expression levels of miR-99b and miR-135b and the immunohistochemical grade classification score of Akt were higher in colorectal cancer, peritoneal carcinomatosis, and liver metastasis than in normal tissues (p < 0.05). MiR-99b expression was highest in CRC, whereas miR-135b expression was highest in peritoneal carcinomatosis (p < 0.05). The expression level of miR-99b decreased and that of miR-135b increased in peritoneal and liver metastases compared with that in the tumor tissue. MiR-99b, Akt, and recurrence were risk factors that affected the overall survival rate in the model of clinical predictions (p = 0.045, p = 0.006, and p = 0.012, respectively).ConclusionWhile the expression of miR-99b was highest in the primary tumor, its decrease in liver metastasis and peritoneal carcinomatosis suggests that miR-99b has a protective effect against liver metastasis and peritoneal carcinomatosis. However, the detection of miR-135b expression was highest in peritoneal carcinomatosis and liver metastasis compared with that in the colorectal cancer tissues suggesting that it facilitates peritoneal carcinomatosis and liver metastasis. Furthermore, miR-99b, KRAS mutations, and Akt are risk factors for the overall survival of colorectal cancer.Copyright © 2023 HCFMUSP. Published by Elsevier España, S.L.U. All rights reserved.

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