• Mol Pain · Mar 2024

    Neurotropin® ameliorates chronic pain associated with scar formation in a mouse model: A gene expression analysis of the inflammatory response.

    • Xuan Zhou, Hiroki Iida, Yugiang Li, Akinobu Ota, Lisheng Zhuo, Reiko Nobuhara, Yuki Terajima, Mitsuru Naiki, A Hari Reddi, Koji Kimata, and Takahiro Ushida.
    • Multidisciplinary Pain CenterAichi Medical University.
    • Mol Pain. 2024 Mar 21: 1744806924124542017448069241245420.

    BackgroundScar formation after trauma and surgery involves an inflammatory response and can lead to the development of chronic pain. Neurotropin® (NTP) is a nonprotein extract from inflamed skin of rabbits inoculated with vaccinia virus and has been widely used for the treatment of chronic pain. However, the in vivo effects of NTP on painful scar formation have not been determined. To investigate the molecular mechanisms underlying the effects of NTP on the inflammatory response, we evaluated gene expression in the scar tissues and dorsal root ganglions (DRGs) of mice administered NTP and control mice.Methods And ResultsMice were injected with saline or NTP as controls, whereas the other mice underwent surgery on the left hind paw to induced painful scar formation, followed by injections of saline or NTP. Hind paw pain was evaluated by measuring the threshold for mechanical stimulation using the von Frey test. The paw withdrawal threshold gradually returned to pre-operative levels over four weeks post-operation; NTP-treated mice showed a significantly shortened recovery time of approximately three weeks, suggesting that NTP exerted an analgesic effect in this mouse model. Total RNA was extracted from the scarred hind paw tissues and DRGs were collected 1 week post-operation for a microarray analysis. A gene set enrichment analysis revealed that some gene sets related to inflammatory responses were activated or inhibited following surgery and NTP administration. Quantitative real-time reverse transcription-polymerase chain reaction data for several genes were consistent with the microarray results.ConclusionThe administration of NTP to the hind paws of mice with painful scar formation following surgery diminished nociceptive pain and reduced the inflammatory response. NTP inhibited the expression of some genes involved in the response to surgery-induced inflammation. Therefore, NTP is a potential therapeutic option for painful scar associated with chronic pain.

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