• Adam J Booth, Ryan Hadley, Ashley M Cornett, Alyssa A Dreffs, Stephanie A Matthes, Jessica L Tsui, Kevin Weiss, Jeffrey C Horowitz, Vincent F Fiore, Thomas H Barker, Bethany B Moore, Fernando J Martinez, Laura E Niklason, and Eric S White.
• Division of Pulmonary and Critical Care Medicine, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI 48109-5642, USA.
• Am. J. Respir. Crit. Care Med.. 2012 Nov 1;186(9):866-76.

RationaleExtracellular matrix (ECM) is a dynamic tissue that contributes to organ integrity and function, and its regulation of cell phenotype is a major aspect of cell biology. However, standard in vitro culture approaches are of unclear physiologic relevance because they do not mimic the compositional, architectural, or distensible nature of a living organ. In the lung, fibroblasts exist in ECM-rich interstitial spaces and are key effectors of lung fibrogenesis.ObjectivesTo better address how ECM influences fibroblast phenotype in a disease-specific manner, we developed a culture system using acellular human normal and fibrotic lungs.MethodsDecellularization was achieved using treatment with detergents, salts, and DNase. The resultant matrices can be sectioned as uniform slices within which cells were cultured.Measurements And Main ResultsWe report that the decellularization process effectively removes cellular and nuclear material while retaining native dimensionality and stiffness of lung tissue. We demonstrate that lung fibroblasts reseeded into acellular lung matrices can be subsequently assayed using conventional protocols; in this manner we show that fibrotic matrices clearly promote transforming growth factor-β-independent myofibroblast differentiation compared with normal matrices. Furthermore, comprehensive analysis of acellular matrix ECM details significant compositional differences between normal and fibrotic lungs, paving the way for further study of novel hypotheses.ConclusionsThis methodology is expected to allow investigation of important ECM-based hypotheses in human tissues and permits future scientific exploration in an organ- and disease-specific manner.

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