• Mesut Sengul, Neslihan Hekim, Ramazan Asci, and Sezgin Gunes.
• Ondokuz Mayıs University, Faculty of Medicine, Department of Urology - Samsun, Turkey.
• Rev Assoc Med Bras (1992). 2024 Jan 1; 70 (10): e20240211e20240211.

ObjectiveThis randomized clinical trial study aims to investigate the effects of antioxidant food supplementation on the total antioxidant capacity of seminal plasma, sperm DNA fragmentation, sperm chromatin quality, and semen parameters.MethodsIn this study, a total of 48 subfertile men with moderate physical activity were included. Group 1 was recommended to use the antioxidant supplements, while antioxidant food supplements were not given to Group 2. Total antioxidant capacity, sperm DNA fragmentation, sperm chromatin structure, hormone levels, physical activities, and semen parameters were evaluated before and after treatment. Total antioxidant capacity, sperm DNA fragmentation, and sperm chromatin structure were assessed using ELISA, transferase dUTP nick end labeling, and aniline blue staining, respectively.ResultsSperm DNA fragmentation (p=0.003) and histone/protamine ratio (p<0.001) were significantly decreased in the patients receiving antioxidant treatment. There was no statistical difference in the total antioxidant capacity values of the post-treatment groups.ConclusionAntioxidant therapy seems to improve sperm DNA fragmentation and histone/protamine ratios in subfertile patients.Clinical Trial Registration NumberNCT06042738.

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