• Norifumi Matsuda, Chigusa Sato, Kosuke Ishikawa, Takahiro Miura, Emi Funayama, Yuhei Yamamoto, Kanako Fuyama, Shunsuke Ohnishi, and Taku Maeda.
• Department of Plastic and Reconstructive Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Kita 15, Nishi 7, Kita-ku, Sapporo, Hokkaido 060-8638, Japan.
• Burns. 2024 Dec 10; 51 (2): 107348107348.

IntroductionThe pathophysiology of burn wound conversion is not fully understood. Animal models are needed to elucidate the underlying mechanisms and develop treatments. Here, we established a new reproducible mouse model that simulates this process, thereby facilitating studies of burn wound conversion.Materials And MethodsAfter anesthesia and depilation, 75 mice were randomly assigned to 5-, 15-, and 25-s contact groups, and a top hat-shaped brass template was heated in boiling water and applied to the lateral abdomen. The wound area was calculated from photographs and the percentage of the surviving area was determined. Histological samples were collected 1-96 h after injury.ResultsA 15-s contact time produced the desired 50 %-75 % interspace necrosis at 96 h after injury. The 5-s contact group had a mostly preserved interspace, while the 25-s contact group exhibited near-complete necrosis. Histologically, significant differences between the 5- and 15-s contact groups were seen in cutaneous appendage denaturation and panniculus carnosus denaturation early after injury.ConclusionExposing mice to the heated template for 15 s provides a reproducible model for studying burn wound conversion mechanisms and treatments, facilitating further elucidation of burn pathophysiology and evaluation of therapies to prevent burn wound conversion.Copyright © 2024 Elsevier Ltd and International Society of Burns Injuries. All rights reserved.

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