• Manuella Pinto Kaster, Angelo Oscar Rosa, Adair R S Santos, and Ana Lúcia S Rodrigues.
• Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário Trindade - 88040-900, Florianópolis-SC, Brasil.
• Int. J. Neuropsychopharmacol. 2005 Dec 1;8(4):601-6.

AbstractWe have previously shown that an acute administration of adenosine produces an antidepressant-like effect in the forced swimming test (FST) and in the tail suspension test in mice. In this work we investigated the contribution of the nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway to adenosine's antidepressant-like effect in the FST since this signalling pathway is assumed to play an important role in depression. The effect of adenosine (10 mg/kg i.p.) was prevented by pre-treatment with L-arginine (750 mg/kg i.p.), S-nitroso-N-acetyl-penicillamine (SNAP, 25 microg/site i.c.v), or sildenafil (5 mg/kg i.p.), but not with D-arginine (750 mg/kg i.p.). Treatment of mice with N(G)-nitro-L-arginine ( L-NNA, 0.03 and 0.3 mg/kg i.p.), Methylene Blue (18 mg/kg i.p.), or ODQ (30 pmol/site i.c.v.) potentiated the effect of adenosine (1 mg/kg i.p.) in the FST. The reduction of immobility time elicited by adenosine (10 mg/kg i.p.) in the FST was prevented by pre-treatment with sildenafil (0.5 and 5 mg/kg i.p.). Together the results indicate that the effect of adenosine in the FST appears to be mediated through an interaction with the NO-cGMP pathway.

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