• Shaofan Ke, Feng Liu, and Zhuanghui Zhu.
• Department of Anesthesiology, First Hospital, Xiamen University, Xiamen Fujian 361000, China.
• Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2014 Dec 1;39(12):1259-65.

ObjectiveTo explore whether resveratrol can reduce intestinal damage in hemorrhagic shock rats and the underlying mechanism.MethodsA total of 24 Sprague-Dawley rats of specifi c pathogen free (SPF) were randomly divided into a control group(n=8), a resveratrol group (SR group, n=8) and a vehicle group (SS group, n=8). Th e mean arterial pressure was recorded. Two hours aft er hemorrhagic shock, 15 mg/kg resveratrol or 0.3 mL equal volume of vehicle and autologous blood were given, respectively. The intestinal specimens were collected for hematoxylin-eosin (HE) staining and calculated the pathological score. The superoxide dismutase 2 (SOD2) and cytochrome C (Cyt C) protein expression was examined by immunohistochemistry and/or Western blot. ATP level, activities of glutathione peroxidase (GXH-px), catalase (CAT) and SOD were also detected.ResultsTwo hours after autologous blood transfusion, the mean arterial pressure in the SR group was significantly higher than that in the SS group (P< 0.01). Compared with the SS group, the pathological injury was significantly alleviated and pathological scores were dramatically reduced in the SR group (P< 0.05). The activities of GXH-px, CAT, SOD and the ATP levels in the SR group were significantly higher than those in the SS group (all P< 0.01). Compared with the SS group, the SOD2 expression was significantly higher while the Cyt C expression was dramatically lower in the SR group (both P< 0.01).ConclusionResveratrol could alleviate the intestinal injury in hemorrhagic shock rats, which might be associated with its effects on reduction of oxidative stress and protection of mitochondria.

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