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Review Comparative Study
Characterization of proteins by mass spectrometry. Invited lecture.
- P Roepstorff.
- Department of Molecular Biology, Odense University, Denmark.
- Analyst. 1992 Mar 1;117(3):299-303.
AbstractPlasma desorption and fast atom bombardment mass spectrometry have in the last decade demonstrated the potential of mass spectrometry for protein studies. The recently developed matrix-assisted laser desorption and electrospray mass spectrometry have expanded the analytical potential of mass spectrometry to cover nearly all proteins. The type of information obtained with the four methods is described and their performances are compared. The potential of combining mass spectrometric relative molecular mass information on proteins with the information contained in protein sequence databases is outlined and some typical fields of application of mass spectrometry in protein chemistry are described. The need for the full integration of mass spectrometry in the protein laboratory is discussed.
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