• Nak-Hyun Kim, Moonsuk Kim, Shinwon Lee, Na Ra Yun, Kye-Hyung Kim, Sang Won Park, Hong Bin Kim, Nam-Joong Kim, Eui-Chong Kim, Wan Beom Park, and Myoung-Don Oh.
• Department of Internal Medicine, Seoul National University College of Medicine, 28 Yongon-Dong, Chongro-Gu, Seoul, Republic of Korea.
• Ann. Intern. Med. 2011 Feb 1; 154 (3): 145-51.

BackgroundBlood culture contamination leads to inappropriate or unnecessary antibiotic use. However, practical guidelines are inconsistent about the routine use of sterile gloving in collection of blood for culture.ObjectiveTo determine whether the routine use of sterile gloving before venipuncture reduces blood culture contamination rates.DesignCluster randomized, assessor-blinded, crossover trial (ClinicalTrials.gov registration number: NCT00973063).SettingSingle-center trial involving medical wards and the intensive care unit.Participants64 interns in charge of collection of blood for culture were randomly assigned to routine-to-optional or optional-to-routine sterile gloving groups for 1854 adult patients who needed blood cultures.InterventionDuring routine sterile gloving, the interns wore sterile gloves every time before venipuncture, but during optional sterile gloving, sterile gloves were worn only if needed.MeasurementsIsolates from single positive blood cultures were classified as likely contaminant, possible contaminant, or true pathogen. Contamination rates were compared by using generalized mixed models.ResultsA total of 10 520 blood cultures were analyzed: 5265 from the routine sterile gloving period and 5255 from the optional sterile gloving period. When possible contaminants were included, the contamination rate was 0.6% in routine sterile gloving and 1.1% in optional sterile gloving (adjusted odds ratio, 0.57 [95% CI, 0.37 to 0.87]; P = 0.009). When only likely contaminants were included, the contamination rate was 0.5% in routine sterile gloving and 0.9% in optional sterile gloving (adjusted odds ratio, 0.51 [CI, 0.31 to 0.83]; P = 0.007).LimitationBlood cultures from the emergency department, surgical wards, and pediatric wards were not assessed.ConclusionRoutine sterile gloving before venipuncture may reduce blood culture contamination.

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