• Rodney D Bell, Glenn D Frazer, David G Brock, Jamie Strause, Barbara L Powers, Timothy J Pelura, Michael S Kramer, and Jewell L Osterholm.
• Department of Neurology, Thomas Jefferson University, 1025 Walnut Street, 310 College Building, Philadelphia, PA 19107, USA.
• Crit. Care Med. 2002 Dec 1; 30 (12): 2684-8.

ObjectiveTo ascertain whether ventriculosubarachnoid perfusion of the brain with an oxygen-carrying nutrient emulsion affects the volume of infarction in animals with permanent middle cerebral artery occlusion.DesignProspective, randomized, controlled trial.SettingAn animal laboratory in a university setting.SubjectsTwenty-eight closed colony adult cats weighing between 3.5 and 4.5 kg.InterventionsCats were assigned randomly into one of three groups: untreated surgical controls, artificial cerebrospinal fluid (ACSF) perfused, or oxygenated fluorochemical (t-bis perfluorobutylethylene; F44E) nutrient emulsion (OFNE) perfused. The formulation used in this study was developed for clinical use and is currently being used in a phase 1 clinical trial in patients with severe ischemic stroke. Focal cerebral ischemia was induced by permanently clipping the middle cerebral artery via the retro-orbital approach. Treatment was initiated 90 mins postocclusion and continued for 18 hrs. Animals were killed 1 hr after the termination of perfusion, the brains were sectioned and stained with 2,3,5-triphenyltetrazolium chloride, and the infarct area was determined with a computer digitizer.Measurements And Main ResultsThere was a significant difference in cerebral infarct volume in the OFNE-perfused animals compared with the other groups ( pConclusionsFluorochemical emulsion delivery of oxygen and nutrients directly to the brain by means of ventriculocisternal perfusion is a viable treatment alternative for cerebral ischemia. In addition, this technique may control elevations in intracranial pressure following stroke.

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