• J. Pharmacol. Sci. · Jan 2010

    Sodium-calcium exchangers in rat ameloblasts.

    • Reijiro Okumura, Yoshiyuki Shibukawa, Takashi Muramatsu, Sadamitsu Hashimoto, Kan-Ichi Nakagawa, Masakazu Tazaki, and Masaki Shimono.
    • Oral Health Science Center, hrc7, Tokyo Dental College, Mihama-ku, Chiba 261-8502, Japan.
    • J. Pharmacol. Sci. 2010 Jan 1; 112 (2): 223-30.

    AbstractAlthough the central role of ameloblasts in synthesis and resorption of enamel matrix proteins during amelogenesis is well documented, the Ca(2+)-transport/extrusion mechanism remains to be fully elucidated. To clarify Ca(2+)-transport in rat ameloblasts, we investigated expression and localization of Na(+)-Ca(2+) exchanger (NCX) isoforms and the functional characteristics of their ion transporting/pharmacological properties. RT-PCR and immunohistochemical analyses revealed expression of NCX1 and NCX3 in ameloblasts, localized in the apical membrane. In patch-clamp recordings, Ca(2+) efflux by Na(+)-Ca(2+) exchange showed dependence on external Na(+). Ca(2+) influx by Na(+)-Ca(2+) exchange, measured by fura-2 fluorescence, showed dependence on extracellular Ca(2+) concentration, and it was blocked by NCX inhibitors KB-R7943, SEA0400, and SN-6. These results showed significant expression of NCX1 and NCX3 in ameloblasts, indicating their involvement in the directional Ca(2+) extrusion pathway from cells to the enamel mineralizing front.

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