• Ayantika Talukdar, Reeta Rai, K Aparna Sharma, D N Rao, and Alpana Sharma.
• Department of Biochemistry, All India Institute of Medical Sciences, AIIMS, New Delhi 110029, India.
• Cytokine. 2018 Feb 1; 102: 117-122.

BackgroundGamma delta (γδ) T cells are known to link innate and adaptive immunity. Decidual γδ T cells are known to provide immunotolerance by producing IL-10 and TGF-β. In recurrent pregnancy loss (RPL) females, the role of peripheral γδ T cells remain unstudied.ObjectiveTo investigate the different phenotypes of γδ T cells in the peripheral blood of women with idiopathic RPL and their possible involvement in RPL condition.MethodsA total of 120 women were recruited for the study. Peripheral blood lymphocytes were isolated and they were stained with appropriate antibodies to determine the phenotype of γδ T cells and major cytokines produced by them in the blood using flow cytometry.ResultsWe observed a significant decrease in the proportion of CD3+CD4-CD8-γδ T cells (p<0.001) and increase in the percentage of IFN-γ (p<0.05) and IL-17 (p<0.001) producing γδ T cells in RPL pregnant as compared to normal pregnant females.ConclusionIncrease in IFN-γ and IL-17-producing CD3+ CD4-CD8- γδ T cells is associated with creating inflammatory cytokine milieu, thereby, may contribute towards pregnancy loss in RPL females.Copyright © 2017 Elsevier Ltd. All rights reserved.

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