• V Weisbach, W Riego, E Strasser, J Zingsem, J Ringwald, R Zimmermann, and R Eckstein.
• Department of Transfusion Medicine and Haemostaseology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany. Volker.Weisbach@trans.imed.uni-erlangen.de
• Vox Sang. 2004 Jul 1; 87 (1): 19-26.

Background And ObjectivesNo data are currently available on the quality of washed prestorage leucocyte-depleted red blood cell concentrates (RCCs).Materials And MethodsFive groups of RCCs stored in additive solution (SAG-M) were washed. The groups differed in the age of RCCs (2-5 days or 11-15 days), the temperature during the washing procedure and a 6-h storage period (4 degrees C or room temperature) and the washing solution (saline, SAG-M or 5% albumin). We measured ATP, 2,3-diphosphoglycerate (2,3-DPG), haemolysis, blood cell count, Na(+), K(+), pH, pO(2), pCO(2) and lactate, before and after the washing procedure and hourly during the 6-h postwash storage period.ResultsThe erythrocyte ATP content increased by 2-13%, relative to the baseline value, during the washing procedure. The 2,3-DPG level decreased by 15-35% in 2-6-day-old RCCs and by 30-40% in 11-15-day-old RCCs (relative to baseline values) during the washing procedure. In RCCs that were washed and stored at room temperature, and in 2-week-old RCCs, a further decrease in 2,3-DPG of up to 40%, relative to the baseline value, was observed during the 6-h postwash time-period.ConclusionsWashing of RCCs stored in SAG-M results in a considerable, significant loss of erythrocyte 2,3-DPG, especially in older RCCs. This loss increases in during a 6-h storage period postwash, even at 4 degrees C. This loss of erythrocyte quality might well outweigh the benefits of washed SAG-M RCCs during massive transfusion in neonates.

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