Progress in molecular biology and translational science
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Prog Mol Biol Transl Sci · Jan 2020
ReviewIn vitro models for ASD-patient-derived iPSCs and cerebral organoids.
Autism spectrum disorder (ASD) is a set of pervasive neurodevelopmental disorders. The causation is multigenic in most cases, which makes it difficult to model the condition in vitro. ⋯ This chapter aims to give an overview of the iPSC technology for generating neural cells and cerebral organoids as models for neurodevelopment and how these models are utilized in the study of ASD. The combination of iPSC technology and the genetic modification tool CRISPR/Cas9 is described, and current limitations and future perspectives of iPSC technology is discussed.