Applied optics
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A new technique for parallel recording of reflection photoplethysmography (PPG) signals in a broad spectral band (violet to near-infrared) has been developed, and its potential for assessment of blood microcirculation at various depths from the skin surface is discussed. PPG signals have been simultaneously detected at cw laser wavelength sets comprising 405, 532, 645, 807, and 1064 nm. Various signal baseline responses to breath holding and different shapes of the PPG pulses originated from the same heartbeat but recorded at different wavelengths have been observed, indicating a depth variety of the skin blood pulsation dynamics.
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We present an optimized linear algorithm for the spatial nonuniformity correction of a CCD color camera's imaging system and the experimental methodology developed for its implementation. We assess the influence of the algorithm's variables on the quality of the correction, that is, the dark image, the base correction image, and the reference level, and the range of application of the correction using a uniform radiance field provided by an integrator cube. The best spatial nonuniformity correction is achieved by having a nonzero dark image, by using an image with a mean digital level placed in the linear response range of the camera as the base correction image and taking the mean digital level of the image as the reference digital level. The response of the CCD color camera's imaging system to the uniform radiance field shows a high level of spatial uniformity after the optimized algorithm has been applied, which also allows us to achieve a high-quality spatial nonuniformity correction of captured images under different exposure conditions.
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There is a growing need for sky radiometric measurements that encapsulate spatial as well as temporal variability. Since the advent of fast data acquisition systems in the 1980s, recent studies have utilized radiation filter instruments deployed in various sky-shading platforms. ⋯ We provide a methodology for a determination of the sensor sky-view factor by using the employed shading device and the sun as a light source. The effect of an incorrect determination of instrument sky-view factor is also analyzed in context of the measurements.
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A novel imaging spectrometer can individually control spatial and spectral resolution by using zoom lenses as the foreoptics of the system and a focusing lens. By varying the focal length we can use the focusing lens to change the spatial and spectral dimensions; with the foreoptics, however, we can change only the spatial dimension. Therefore the spectral resolution and the spectral range are affected by the zoom ratio of the focusing lens, whereas the spatial resolution and the field of view are affected by the multiplication of the zoom ratios of the foreoptics and the focusing lens. ⋯ For an imaging spectrometer with this novel zooming function, we used the lens module method and third-order aberration theory to design an initial four-group zoom system with an external entrance pupil for the focusing lens. Furthermore, using the optical design software CODE V, we obtained an optimized zoom lens with a focal-length range of 50 to 150 mm. Finally, the zoom system with its transmission grating in the Littrow configuration performs satisfactorily as the focusing lens of an imaging spectrometer in the wavelength range 450-900 nm.
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We apply the techniques of digital holography to obtain microscopic three-dimensional images of biological cells. The optical system is capable of microscopic holography with diffraction-limited resolution by projecting a magnified image of a microscopic hologram plane onto a CCD plane. ⋯ The method of three-wavelength phase imaging is proposed to extend the axial range and reduce the effect of phase noise. These results demonstrate the effectiveness of digital holography in high-resolution biological microscopy.