Carbohydrate polymers
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Carbohydrate polymers · Nov 2015
Development of silver sulfadiazine loaded bacterial cellulose/sodium alginate composite films with enhanced antibacterial property.
Sodium alginate (SA) and bacterial cellulose (BC) are widely used in many applications such as scaffolds and wound dressings due to its biocompatibility. Silver sulfadiazine (AgSD) is a topical antibacterial agents used as a topical cream on burns. In the study, novel BC/SA-AgSD composites were prepared and characterized by SEM, FTIR and TG analyses. ⋯ The antibacterial performances of BC/SA-AgSD composites were evaluated with Escherichia coli, Staphylococcus aureus and Candida albicans. Moreover, the cytotoxicity of BC/SA-AgSD composites was performed on HEK 293 cells. The experimental results showed BC/SA-AgSD composites have excellent antibacterial activities and good biocompatibility, thus confirming its utility as potential wound dressings.
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Carbohydrate polymers · Oct 2014
Antitumor metastasis activity of pectic polysaccharide purified from the peels of Korean Citrus Hallabong.
A polysaccharide fraction, HBE-III, was successfully purified in a high yield (40.4%) from its crude polysaccharide (HBE-0) which was prepared from pectinase hydrolysates of the peels of the Korean Citrus Hallabong. In experimental lung metastasis studies of Colon 26-M3.1 carcinoma cells, prophylactic administration of HBE-III significantly inhibited lung metastasis in a dose-dependent manner. In an in vitro cytotoxicity analysis, HBE-III (up to 1000 μg/mL) did not affect the growth of Colon 26-M3.1 cells and normal cells. ⋯ In an assay for natural killer (NK) cell activity, HBE-III (1000 μg/mouse, i.v.) significantly augmented NK cytotoxicity against Yac-1 tumor cells. The depletion of NK cells by injection of mouse anti-asialo GM1 serum abolished the inhibitory effect of HBE-III on lung metastasis of Colon 26-M3.1 cells. These data suggest that HBE-III may inhibit tumor metastasis via activation of macrophages and NK cells.
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Carbohydrate polymers · Feb 2014
Preparation of tamarind gum based soft ion gels having thixotropic properties.
Tamarind gum was used to prepare ion gels using both synthetic ionic liquids (ILs) namely 1-butyl-3-methylimidazolium chloride and 1-butyl-3-methylimidazolium bromide and bio-based ionic liquids (Bio-ILs) namely choline acrylate, choline caproate and choline caprylate by heating cooling process. The gels were found to have good thermal stability and exhibited thixotropic behaviour. ⋯ The ion gels also showed very good adherence to human finger muscles and skin. The ion gels thus prepared may find application in electrochemistry, sensors, actuators and the gels prepared with Bio-ILs could even be useful in biomedical applications.
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Carbohydrate polymers · Feb 2014
Montmorillonite-chitosan-silver sulfadiazine nanocomposites for topical treatment of chronic skin lesions: in vitro biocompatibility, antibacterial efficacy and gap closure cell motility properties.
Silver compounds and especially silver sulfadiazine (AgSD) are reported as effective antimicrobial agents against almost all known bacteria, fungi and some viruses. However, AgSD has been shown to be cytotoxic toward fibroblasts and keratinocytes in vitro and consequently to retard wound healing in vivo. The aim of the present work was to evaluate the in vitro biocompatibility (cytotoxicity and proliferation), antimicrobial efficacy and cell motility gap closure (assay of wound closure) of MT/CS nanocomposites loaded with silver sulfadiazine (AgSD). ⋯ The nanocomposite prepared using 100mg of MT was characterized for in vitro biocompatibility and proliferation and for wound healing using normal human dermal fibroblasts (NHDF). Antimicrobial properties were evaluated against four reference bacterial strains: Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa. AgSD loaded in the 100 MT/CS nanocomposite showed good in vitro biocompatibility and gap closure properties (fibroblasts) and maintained AgSD antimicrobial properties, especially against P. aeruginosa, that often complicates skin lesions.
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Carbohydrate polymers · Feb 2013
Polysaccharide glucomannan isolated from Heterodermia obscurata attenuates acute and chronic pain in mice.
Glucomannan (GM) is a polysaccharide obtained from Heterodermia obscurata lichens. The present study was conducted to elucidate the antinociceptive effect of GM in behavioural models of acute and chronic pain in mice. ⋯ Systemic treatment with GM inhibited the nociception induced by intraplantar injection of glutamate and by intrathecal injection of N-methyl-d-aspartic acid (NMDA), (±)-1-aminocyclopentane-trans-1,3-dicarboxylic acid (trans-ACPD), tumour necrosis factor α (TNF-α) and IL-1β. Taken together, our data demonstrate that GM has significant antinociceptive effect in acute and chronic pain, suggesting a potential interest in the development of new clinically relevant drugs for the management of pain.