Journal of clinical laboratory analysis
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J. Clin. Lab. Anal. · Jan 2011
Comparative StudyDevelopment of simultaneous detection of total prostate-specific antigen (tPSA) and free PSA with rapid bead-based immunoassay.
Prostate-specific antigen (PSA) is the most important biochemical tumor marker for the early detection of prostate cancer; however, its diagnostic specificity is low. Therefore, free PSA (fPSA) test is recommended as an adjunct to increase the specificity. However, all the current technology only allows detecting one biomarker at one time. In this study, we reported a flexible bead-based immunoassay to measure total PSA (tPSA) and fPSA simultaneously. ⋯ The bead-based platform is rapid, sensitive, and less expensive, which allows both single sample and high-throughput measurement of tPSA and fPSA over a wide range of concentrations.
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J. Clin. Lab. Anal. · Jan 2011
Ischemia-modified albumin as a biochemical marker in children with neuroblastoma and soft tissue sarcomas.
In this study, the levels of ischemia-modified albumin (IMA) in pediatric oncology patients with soft tissue sarcomas (STSs) and neuroblastoma (NB) were analyzed. To date, there have been no studies concerning IMA in these groups of patients. Ninety-nine children with STSs and NB were analyzed from 2006 to 2009, and 30 healthy children were also enrolled in the study. ⋯ The levels of IMA in all cancer patients (mean 116.8±39.3 U/ml), in patients with STSs (mean 119.8±27.5 U/ml), and in patients with NB (mean 114.6±36.6 U/ml) were significantly higher than in the control patients (mean 87.3±38.3 U/ml; P=0.0013, 0.0066, and 0.0164, respectively). IMA levels increased before and during the treatment compared with levels in the controls. The determination of IMA levels in pediatric oncology patients with poor prognoses from STSs and NB may play an important role in predicting response to therapy and overall outcome.
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J. Clin. Lab. Anal. · Jan 2010
Comparative StudyRole of tissue disorder markers in the evaluation of disease progress and outcome prediction: a prospective cohort study in non-cardiac critically ill patients.
Acute Physiology and Chronic Health Evaluation (APACHE) has a remarkable association with clinical syndrome, life expectancy, and length of ward stay. But the defects are obvious. It is crucial to detect an effective and convenient evaluation method to monitor disease progress and predict outcome. ⋯ All the six tissue disorder markers are predictors of disease severity, organ failure, and outcome in non-cardiac critically illness. Among them, Mb plays a pivotable role. The combined use of Mb and APACHE-II suggest an effective method to determine the outcome of critical ill syndrome.
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J. Clin. Lab. Anal. · Jan 2010
Comparative StudyPredictive values of neutrophil CD64 expression compared with interleukin-6 and C-reactive protein in early diagnosis of neonatal sepsis.
Despite major advances in the management of newborn infants, neonatal sepsis (NS) remain important causes of neonatal morbidity and mortality in the newborn, mainly among preterm and low birth weight infants. ⋯ CD64 might be used either alone or combined with IL-6 and CRP for early diagnosis of NS. The advantages of CD64 when compared with IL-6 and CRP are rapid quantitation, very small blood volume required, and easy handling.
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J. Clin. Lab. Anal. · Jan 2010
Alterations in biomarkers of endothelial function following on-pump coronary artery revascularization.
Cardiopulmonary bypass (CPB) has been associated with activation and injury of endothelial cells, probably responsible for the systemic inflammatory response syndrome (SIRS) taking place in these patients. ⋯ CPB is characterised by pronounced changes in plasma sP-s, sE-s, TN, vWF levels, and ACE activity, which are associated with significant alteration in the intra- and early postoperative endothelial function observed in open heart surgery.