The journals of gerontology. Series A, Biological sciences and medical sciences
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J. Gerontol. A Biol. Sci. Med. Sci. · Jun 2010
Comparative StudyDevelopment and validation of a video-animated tool for assessing mobility.
Existing self-report measures of mobility ignore important contextual features of movement and require respondents to make complex judgments about specific tasks. Thus, we describe the development and validation of a short form (sf) video-animated tool for assessing mobility, the Mobility Assessment Tool-MAT-sf. ⋯ The MAT-sf is an innovative psychometrically sound measure of mobility. It has utility in epidemiological studies, translational science, and clinical practice.
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J. Gerontol. A Biol. Sci. Med. Sci. · Jun 2010
Age-associated differences in activation of Akt/GSK-3beta signaling pathways and inhibition of mitochondrial permeability transition pore opening in the rat heart.
Pretreatment with isoflurane decreased myocardial infarction size in young rats (3-5 months) but not in old rats (20-24 months). To understand the mechanisms underlying the failure to protect the old myocardium, differences in phosphorylation of Akt/GSK-3beta and age-associated differences in mitochondrial permeability transition pore (mPTP) opening in the aging heart in vivo were measured. Isoflurane significantly increased Akt and GSK-3beta phosphorylation in the young groups. ⋯ Isoflurane preconditioning significantly reduced the fall in NAD(+) levels induced by ischemia/reperfusion injury in the young animals, reflecting the inhibition of mPTP opening. In the old animals, however, isoflurane failed to prevent the fall in NAD(+) levels induced by ischemia/reperfusion injury. Lack of isoflurane-induced cardioprotective effects, seen in the old animals, can be explained by age-related differences in Akt/GSK-3beta signaling pathway and the inability to reduce mPTP opening following ischemia/reperfusion injury.