Journal of virology
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Journal of virology · Aug 2002
Identification of an upstream sequence element required for vesicular stomatitis virus mRNA transcription.
Vesicular stomatitis virus (VSV), the prototypic rhabdovirus, has a nonsegmented negative-sense RNA genome with five genes flanked by 3' leader and 5' trailer sequences. Transcription of VSV mRNAs is obligatorily sequential, starting from a single 3' polymerase entry site, and termination of an upstream mRNA is essential for transcription of a downstream gene. cis-acting signals for transcription of VSV mRNAs are present within the leader region, at the leader-N junction, and at the internal gene junctions. The gene junctions of VSV consist of a conserved 23-nucleotide region that includes the gene end sequence of the upstream gene, 3'-AUACU7-5', a nontranscribed intergenic dinucleotide, 3'-G/CA-5', and the gene start sequence, 3'-UUGUCNNUAG-5', at the beginning of the gene immediately downstream. ⋯ Our data show that the U7 tract of the upstream gene end sequence is necessary for optimal transcription of the downstream gene, independent of its role in termination of the upstream gene. Altering the sequence or changing the length of the U tract directly upstream of the gene start sequence significantly decreased transcription of the downstream gene. These results show that the U tract is a multifunctional region that is required not only for polyadenylation and termination of the upstream mRNA but also for efficient transcription of the downstream gene.