• Nutrition · Feb 2015

    Selenium reduces bradykinesia and DNA damage in a rat model of Parkinson's disease.

    • Joel Henrique Ellwanger, Patrícia Molz, Danieli Rosane Dallemole, Ariana Pereira dos Santos, Talise Ellwanger Müller, Lucas Cappelletti, Manoela Gonçalves da Silva, Silvia Isabel Rech Franke, Daniel Prá, and João Antonio Pêgas Henriques.
    • Graduate Program in Cellular and Molecular Biology, Federal University of Rio Grande do Sul-UFRGS, Porto Alegre, RS, Brazil; Laboratory of Histology and Pathology, University of Santa Cruz do Sul-UNISC, Santa Cruz do Sul, RS, Brazil; Laboratory of Experimental Nutrition, University of Santa Cruz do Sul-UNISC, Santa Cruz do Sul, RS, Brazil. Electronic address: Joelellwanger@unisc.br.
    • Nutrition. 2015 Feb 1;31(2):359-65.

    ObjectiveThe aim of this study was to explore the effects of selenium (Se) on locomotor activity and DNA damage in a rat model of Parkinson's disease (PD) induced by paraquat (PQ).MethodsForty-eight male Wistar rats were divided into four groups: control group (n = 12), Se group (n = 12), PQ group (n = 12), and Se + PQ group (n = 12). PQ was administered intraperitoneally (10 mg/kg). Se was offered in the drinking water at a concentration of 11.18 μg/L. Locomotor activity was evaluated weekly using the narrow beam test. The comet assay was performed to assess the level of DNA damage in leukocytes and in brain cells.ResultsAs expected, increased DNA damage was found in the PQ group compared with the control and Se groups (P < 0.001). Interestingly, coadministration of Se and PQ effectively prevented the harmful effects of the toxin in locomotor activity and at the molecular level, reducing bradykinesia (P < 0.01) and DNA damage in leukocytes compared with the PQ-only group (P < 0.001), whereas the levels of DNA damage were comparable to those found in the control and Se groups (P > 0.05). Using the comet assay to analyze brain cells, no differences were found between the groups with regard to damage index (P = 0.774), damage frequency (P = 0.817), or non-detectable cell nuclei (P = 0.481).ConclusionIn this experimental model of PQ-induced PD, the use of Se could contribute to the maintenance of locomotor activity and the integrity of leukocytes DNA. No changes in the levels of DNA damage in brain cells were observed between the experimental groups.Copyright © 2015 Elsevier Inc. All rights reserved.

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