• M Sh Avruschenko and I V Ostrova.
• V.A. Negovsky Research Institute of General Reanimatology, Moscow, Russia.
• Shock. 2015 Oct 1;44 Suppl 2:19.

IntroductionTo evaluate the significance of GDNF protein expression for developing a neuronal death after ischemia-reperfusion.MethodsAdult white rats both sexes underwent 10-min circulatory arrest evoked by clamping of cardiac vascular bundle, followed by resuscitation. The total number of hypoxia-sensitive cerebellar Purkinje cells (PC) was determined at 1, 4, 7 or 14 day post-resuscitation. The expression of GDNF protein was detected by immunohistochemistry.ResultsIt was revealed elevation of GDNF expression in PC population of males 1 day after resuscitation: the number of positive neurons increased by 70.0%, while the number of negative neurons decreased by 35.4%. The total number of PC was as in control. At 4th day GDNF expression reduced: the number of positive neurons fell to the control and the number of negative neurons decreased by 32.3%. Reducing the GDNF expression was accompanied by neuronal loss (the total number of PC decreased by 16.6%). Later GDNF expression was not changed, and no further death of PC were revealed. We found similar changes in females. But GDNF expression level shifts, as well as neuronal loss developed later than in males.ConclusionThe results demonstrated association between the changes in GDNF protein level and the development of neuronal death process in the post-resuscitation period. Initial rise of GDNF expression in neuronal populations may prevent neuronal loss. The decrease of GDNF expression was accompanied by the neuronal death. GDNF protein level was shown to be an important factor influencing the resistance of neurons to ischemia- reperfusion.

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