• Bioinformatics · Mar 2018

    Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1.

    • Jeongbin Park and Sangsu Bae.
    • Department of Chemistry, Hanyang University, Seoul 04763, South Korea.
    • Bioinformatics. 2018 Mar 15; 34 (6): 1077-1079.

    SummaryFollowing the type II CRISPR-Cas9 system, type V CRISPR-Cpf1 endonucleases have been found to be applicable for genome editing in various organisms in vivo. However, there are as yet no web-based tools capable of optimally selecting guide RNAs (gRNAs) among all possible genome-wide target sites. Here, we present Cpf1-Database, a genome-wide gRNA library design tool for LbCpf1 and AsCpf1, which have DNA recognition sequences of 5'-TTTN-3' at the 5' ends of target sites. Cpf1-Database provides a sophisticated but simple way to design gRNAs for AsCpf1 nucleases on the genome scale. One can easily access the data using a straightforward web interface, and using the powerful collections feature one can easily design gRNAs for thousands of genes in short time.Availability And ImplementationFree access at http://www.rgenome.net/cpf1-database/.Contactsangsubae@hanyang.ac.kr.

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