• Tomoyuki Ozawa, Seiji Ohtori, Gen Inoue, Yasuchika Aoki, Hideshige Moriya, and Kazuhisa Takahashi.
• From the Department of Orthopedic Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan. tomokun@wg7.so-net.ne.jp
• Spine. 2006 Oct 1; 31 (21): 2418-22.

Study DesignImmunohistochemical study of the sensory innervation of the human lumbar intervertebral disc.ObjectiveTo determine the type of sensory fibers innervating human degenerated lumbar intervertebral discs.Summary Of Background DataSensory neurons involved in pain perception related to inflammation in rats are typically small, peptide-containing neurons immunoreactive for calcitonin gene-related peptide (CGRP). Small non-peptide-containing neurons binding to isolectin B4 (IB4) may also be involved in pain states, such as nerve injury pain. The character of such sensory neurons in humans has not been clarified.MethodsA degenerated, painful lumbar intervertebral disc was harvested from each of 8 patients during surgery. Sections were immunostained for protein gene product 9.5 (PGP 9.5, a general neuronal marker), CGRP, and IB4. The numbers of PGP 9.5- and CGRP-immunoreactive, and IB4-binding nerve fibers in the discs were counted.ResultsPGP 9.5-immunoreactive fibers were observed in all discs. Nerve fibers immunoreactive for CGRP were also observed in 6 of 8 cases. IB4-binding nerve fibers were not found in any case.ConclusionsAlmost all of the nociceptive nerve fibers in the human intervertebral disc are peptide-containing nerve fibers, similar to the rat disc, suggesting that nerve fibers related to inflammation may transmit pain originating from human degenerated intervertebral discs.

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