• Qiang Ye, Jiaqi Wang, Xiangwen Liu, Zihua Liu, LuoSong BaZong, Jinhai Ma, Rong Shen, Weichun Ye, Wenfang Zhang, and Degui Wang.
• Institute of Human Anatomy and Histoembryology, Basic Medical College, Lanzhou University, 199 Donggang West Road, Lanzhou 730000, China; Department of Ophthalmology, The Second Hospital of Lanzhou University, 82 Cuiying Door, Lanzhou 730000, China.
• Neuroscience. 2022 Jan 1; 480: 19-31.

AbstractRAD6B is an E2 ubiquitin-conjugating enzyme, playing an important role in DNA damage repair, gene expression, senescence, apoptosis and protein degradation. However, the specific mechanism between ubiquitin and retinal degeneration requires more investigation. Pigment epithelium-derived factor (PEDF) has a potent neurotrophic effect on the retina, protecting retinal neurons and photoreceptors from cell death caused by pathological damage. In this study, we found that loss of RAD6B leads to retinal degeneration in mice, especially in old age. Affymetrix microarray analysis showed that the PEDF signal was changed in RAD6B deficient groups. The expression of γ-H2AX, β-Gal, P53, Caspase-3, P21 and P16 was increased significantly in retinas of RAD6B knockout (KO) mice. Our studies suggest that RAD6B and PEDF play an important role in the health of retina, whereas the absence of RAD6B accelerates the degeneration.Copyright © 2021 IBRO. Published by Elsevier Ltd. All rights reserved.

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