• Kevin C Gonzalez, Attila Losonczy, and Adrian Negrean.
• Department of Neuroscience, Columbia University, New York, NY, USA; Mortimer B. Zuckerman Mind Brain Behavior Institute, New York, NY, USA. Electronic address: kg2685@columbia.edu.
• Neuroscience. 2022 May 1; 489: 165175165-175.

AbstractMuch of our understanding of dendritic and synaptic physiology comes from in vitro experimentation, where the afforded mechanical stability and convenience of applying drugs allowed patch-clamping based recording techniques to investigate ion channel distributions, their gating kinetics, and to uncover dendritic integrative and synaptic plasticity rules. However, with current efforts to study these questions in vivo, there is a great need to translate existing knowledge between in vitro and in vivo experimental conditions. In this review, we identify discrepancies between in vitro and in vivo ionic composition of extracellular media and discuss how changes in ionic composition alter dendritic excitability and plasticity induction. Here, we argue that under physiological in vivo ionic conditions, dendrites are expected to be more excitable and the threshold for synaptic plasticity induction to be lowered. Consequently, the plasticity rules described in vitro vary significantly from those implemented in vivo.Copyright © 2022 IBRO. Published by Elsevier Ltd. All rights reserved.

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