• Neuroscience · Jun 2013

    Intrathecal application of short interfering RNA knocks down c-jun expression and augments spinal motoneuron death after root avulsion in adult rats.

    • X Cheng, R Fu, M Gao, S Liu, Y-Q Li, F-H Song, I C Bruce, L-H Zhou, and W Wu.
    • Department of Anatomy, Zhong Shan School of Medicine, Sun Yat-Sen University, Guangzhou, China.
    • Neuroscience. 2013 Jun 25;241:268-79.

    AbstractThe immediate-early gene, c-jun, is expressed in spinal motoneurons after spinal root avulsion. The expression of c-jun was suggested to be necessary for motoneuron survival and regeneration after avulsion. In the present study, a small interfering RNA (siRNA) was delivered intrathecally to the injured spinal segments immediately after root avulsion in rats to knock down expression of the c-jun gene in injured spinal motoneurons so as to explore the role of c-jun in the motoneurons in vivo. Our results showed that the siRNA not only inhibited the expression of both c-jun mRNA and protein but also augmented the death of injured motoneurons at day 14 post-injury. These findings indicated that induction of c-jun gene expression plays a pivotal role in the survival of injured motoneurons. Meanwhile, these results suggest that siRNAs applied intrathecally can effectively mediate the expression of the c-jun gene in injured motoneurons.Copyright © 2013 IBRO. Published by Elsevier Ltd. All rights reserved.

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