• Guoqing Wang, Zhihua Li, Shujian Li, Junling Ren, Vigneyshwar Suresh, Dingkang Xu, Weidong Zang, Xianzhi Liu, Wei Li, Huizhi Wang, and Fuyou Guo.
• Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China, 450052.
• Neuroscience. 2019 Sep 1; 415: 135-146.

AbstractDisruption of the blood-brain barrier (BBB) and subsequent neurological deficits are the most severe consequence of intracerebral hemorrhage (ICH). Minocycline has been wildly used clinically as a neurological protective agent in clinical practice. However, the underlying mechanisms by which minocycline functions remain unclear. Therefore, we assessed the influence of minocycline on BBB structure, neurological function, and inflammatory responses in a collagenase-induced ICH model, and elucidated underlying molecular mechanisms as well. Following a single injection of collagenase VII-S into the basal ganglia, BBB integrity was assessed by Evans blue extravasation while neurological function was assessed using an established neurologic function scoring system. Minocycline treatment significantly alleviated the severity of BBB disruption, brain edema, and neurological deficits in ICH model. Moreover, minocycline decreased the production of inflammatory mediators including TNF, IL-6, and MMP-9, by microglia. Minocycline treatment decreased DKK1 expression but increased Wnt1, β-catenin and Occludin, a phenomenon mimicked by DKK1 silencing. These data suggest that minocycline improves the consequences of ICH by preserving BBB integrity and attenuating neurologic deficits in a DKK1-related manner that involves enhancement of the Wnt1-β-catenin activity.Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.

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