Pak J Pharm Sci
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To discuss the expression of T helper cell 17 (Th17) cells and CD4+ CD25+ Foxp3+ regulatory T cells (Treg) in peripheral blood (PB) of patients with acute leukemia (AL), and to explore the relationship between them and disease prognosis. 40 patients diagnosed with acute leukemia in The First Affiliated Hospital of Zhengzhou University from July 2012 to August 2014 were selected as the observation group. Meanwhile, 40 healthy people were taken as the control group. Flow Cytometry Method (FCM) was used to detect the level of Th17 cells and CD4+ CD25+ Foxp3+ cells in peripheral blood of the two groups, and enzyme-linked immuno sorbent assay (ELISA) method was used to test the level of IL17 and TGF-β in peripheral blood of two groups; reverse transcription-polymerase chain reaction (RT-PCR) was adopted to analyze the mRNA levels of RORγT and Foxp3 in peripheral blood. ⋯ In addition, compared with before chemotherapy, the levels of Th17 cells and CD4+ CD25+ Foxp3+ cells, and the serum levels of IL-17 and TGF-β in acute leukemia patients all decreased significantly after 6 months of chemotherapy, and the difference was statistically significant (P<0.001). Th17 cells, CD4+ CD25+ Foxp3+ cells and their secretory proteins IL-17, TGF-β and transcription factors were significantly increased in AL patients. Therefore, regular detection of peripheral blood Th17 and Treg cells, as well as their secretory proteins are useful for monitoring the immune status and prognosis of patients.
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Randomized Controlled Trial
Clinical research on the dexmedetomidine applied for patient-controlled sedation during the lower limbs operation under combined spinal-epidural anesthesia.
To investigate the effect and safety of dexmedetomidine applied for patient-controlled sedation under combined spinal anesthesia. 150 cases who would be implemented lower limbs operations were randomly divided into patient-controlled sedation group (Group PCS) and control group (Group C) and 75 cases for each group. The ages of patients were between 18 and 65 years old and patients were with American Society of Anesthesiologists (ASA) or level. After being implemented combined spinal anesthesia, patients of Group PCS were undergone patient-controlled sedation by using dexmedetomidine (4μg/mL) with 2mL of load quantity, 1.5ml of background infusion quantity, 0.5mL of single dose and 20s of locking time; patients of Group C were constantly infused the normal saline at the rate of 10ml/h by pump injection. ⋯ The effective pressing numbers were 21.00±9. 07times. The patient-controlled dosages were (15.12±3.19) ml; The dosages were 11.29±2.16ml when the level of sedation achieved 3 to 4 scores in Ramsay sedation scores; And the required time to achieve 3 to 4 scores in Ramsay sedation scores was 7.55±1.53 min. In the lower limbs operations, the usage of dexmedetomidine applied for patient-controlled sedation under combined spinal anesthesia can effectively approach to the personalized medicine and is effective in clinical application.
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Inter individual variability in polymorphic UDP-glucuronosyltransferase (UGT2B15) has been associated with varied glucuronidation level. The present project was designed to determine the genetic polymorphism of UDP-glucuronosyltransferase (UGT2B15) and glucuronidation of paracetamol in healthy (male=59 and female=50) population. The association between genotype (UGT2B15) and phenotype (paracetamol glucuronidation) has been evaluated. ⋯ The glucuronidation status was investigated by HPLC analysis of paracetamol. Ratio of paracetamol glucuronide to paracetamol was determined with two antimodes at glucuronidation ratio of 0.3 and 1.8. In our study, 7% and 12% of population was distributed as slow glucuronidators by phenotype and genotype, respectively and association between phenotype and genotype was good for analysis of glucuronidation status as displayed by kappa value (0.792).
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To investigate the effects of the iron chelatordeferoxamine (DFA) on inhibition formicroglia activation and protection of secondary nerve injury after intracerebral hemorrhage (ICH) in rats. The rats were randomly divided into sham operation group, ICH group and DFA treatment group. The collagenase was used to prepare ICH model of basal gangliain rats and 1h after the beginning of the operation, the intraperitoneal injection with DFA was arranged every 12 h and for a total of 7d. ⋯ At the same time, the loss of neurons in the tissue around of the hematoma was significantly reduced and neurological deficit scores were significantly reduced. Iron ions which were sustainedly released by hematoma after ICH can activate the local microglia and cause secondary brain injury. DFA curb excessive activation of microglia and reduce neuronal death of ICH by means of clearinf away iron ions of brain tissue surrounding the hematoma, thus improve secondary neurological dysfunction.
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Comparative Study
Comparison of clinical effect of dopamine and norepinephrine in the treatment of septic shock.
This study aims to compare the clinical effect of dopamine and nor epinephrine in the treatment of septic shock. Fifty cases with septic shock were randomly divided into two groups. Patients in both two groups revived after taking effective liquid. ⋯ The improvement of haemodynamics and microcirculation perfusion indexes were compared between two groups before and after treatment, as well as the improvement of tissue oxygen metabolism. The results demonstrated that, central venous pressure (CVP), mean arterial pressure (MAP), urine volume and central venous oxygen saturation (Scv O2) in both groups before treatment was not statistically significant (P>0.05); 6 h after treatment, CVP, MAP, urine volume and Scv O2 of group NE were higher than group DA; 12h and 24h after treatment, blood lactic acid clearance of group NE was superior than group DA (P<0.05). All the above findings suggested that, both dopamine and nor epinephrine are beneficial to improve microcirculation and tissue oxygen metabolism in the treatment of septic shock, and the clinical effect of nor epinephrine was distinctly better than dopamine.